Citation
Elucidating the impact of metals on the taxonomic diversity and functional activity of bacterial communities within the Chattanooga Fen

Material Information

Title:
Elucidating the impact of metals on the taxonomic diversity and functional activity of bacterial communities within the Chattanooga Fen
Creator:
Foster, Kelsey
Roane, T. M.
Place of Publication:
Denver, CO
Publisher:
University of Colorado Denver
Publication Date:
Language:
English

Notes

Abstract:
Fens are marshy wetlands with an accumulation of partially decomposed organic matter. The Chattanooga Fen, located in southwestern Colorado in the San Juan National Forest, is anthropogenicallyand endogenously impacted by metals, which are toxic to cells at elevated concentrations. The anthropogenic source of metals comes from water which has flown over or through sulfur-bearing minerals exposed to air via mining practices at the Gold Finch Mine located just above the fen. The result of this is known as acid mine drainage (AMD), which is rich in heavy metals and has a net acidity which further dissolves other metals. Natural metal impact is a result of acid rock drainage (ARD) which forms from the natural oxidation of sulfur-bearing minerals. The sediments at the Chattanooga Fen have become the final repository for these metals. This study aims to elucidate the impact of metals in shaping the taxonomic and functional diversity of the microbial communities within the uncharacterized Fen. This will be accomplished through the use of Illumina high-throughput sequencing of extracted 16s rDNA from sediment samples. The samples will be collected utilizing coring at the outflow of the Gold Finch mine, along the AMD effluent, and from naturally metal impacted sediments which are unimpactedby AMD. RNA will also be extracted from these samples, and expression of carbon and nitrogen fixation, sulfate reduction, and polyphosphate metabolism genes will be quantified. The understanding of how metals impact the diversity and functional potential of microorganisms will be essential in influencing future management decisions.
General Note:
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Record Information

Source Institution:
University of Colorado Denver Collections
Holding Location:
Auraria Library
Rights Management:
All applicable rights reserved by the source institution and holding location.

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